CUSTOM PROTEIN SYNTHESIS
Early drug discovery projects require identification of key proteins that play pivotal roles in potential therapeutic pathways.
In order to determine the activity and function of a protein target, it must be analyzed through biophysical and structural characterization in parallel with activity assays. Attaining a sufficient amount of purified protein is a prerequisite to completing this analysis. Helix’s custom protein synthesis services (bacteria, insect, and mammalian expression systems) produce crystallization grade recombinant protein having more than 95% purity suitable for any analytical technique.
Protein Synthesis Timeline
1 week
2 weeks
1 week
2 weeks
2 weeks
1 week
1 week
10 weeks
CUSTOM GENE CONSTRUCT TO PURIFIED PROTEIN
Construct Design
Custom construct design enhances experimental success
Fusion partner and affinity tag selection
Codon optimization
Post-translational modifications
DNA isolation and subcloning expertise
Construct optimization paired with a small-scale pilot expression
2. Protein Expression
Bacterial, mammalian, and insect expression platforms
Small-scale preliminary runs validate constructs & conditions
Scale-up expression tailored to customer requirements
Western Blot analysis
Quick turn-around time for literature/SOP reproduction
Isotopically labeled ( 13C/ 15N; 15N) protein for NMR.
Pf (Pseudomonas fluorescens) expression system via Corteva AgriScience
3. Protein Purification
Custom-made purification schemes to provide high purity and homogeneity
Significant experience with protein complex, monoclonal antibody expression & purification
Quality control including SDS-Page, DLS, ITC, and Mass Spec
Typical turn-around time for full-service protein production is ten weeks
